Mouse APRIL/TNFSF13 Protein (active trimer)
Catalog No: APRIL-MP001
- Species
- Mouse
- Expression System
- HEK293
- Tag
- His, Flag
- Activity
- Activity verified
Product overview
Recombinant Mouse APRIL/TNFSF13 Protein is expressed in HEK293 cells with a His tag and Flag tag at the N-terminus. It contains amino acid residues Ala96-Leu241 (UniProt accession: Q9D777).
Product Details
- Molecular Aliases
- CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2
- Protein Length
- Ala96-Leu241
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 18.53 kDa. Due to glycosylation, the protein migrates to 24-30 kDa based on Bis-Tris PAGE result.
- Purity
- > 90% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS, 8% trehalose (pH 7.4).
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Mouse APRIL on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
The purity of Mouse APRIL is greater than 90% as determined by SEC-HPLC.
The purity of Mouse APRIL is greater than 90% and the molecular weight of this protein is around 68-90 kDa as determined by SEC-MALS.
Immobilized Mouse APRIL, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Mouse BCMA, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA.
Loaded Mouse APRIL, His Tag on Anti-His-Biosensor can bind Human TACI, hFc Tag with an affinity constant of 1.98 nM as determined in BLI assay.
Loaded Mouse APRIL, His Tag on Anti-His-Biosensor can bind Mouse BCMA, hFc Tag with an affinity constant of 0.47 nM as determined in BLI assay.
Background
The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily. Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R.
References
- Bossen C, Schneider P. BAFF, APRIL and their receptors: Structure, function and signaling[J]. Seminars in Immunology, 2006, 18(5):0-275.
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