Mouse APRIL/TNFSF13 Protein
Catalog No: APRIL-MF001
- Species
- Mouse
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Mouse APRIL/TNFSF13 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Ala96-Leu241 (UniProt accession: Q9D777).
Product Details
- Molecular Aliases
- CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2
- Protein Length
- Ala96-Leu241
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 43.20 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Mouse APRIL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse APRIL is greater than 95% as determined by SEC-HPLC.
Immobilized Mouse APRIL, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse BCMA, His Tag with the EC50 of 3.4ng/ml determined by ELISA. (QC Test)
Loaded Mouse BCMA, His Tag on Anti-His-Biosensor can bind Mouse APRIL, hFc Tag with an affinity constant of 7.09 nM as determined in BLI assay.
Background
The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R.
References
- Bossen C, Schneider P. BAFF, APRIL and their receptors: Structure, function and signaling[J]. Seminars in Immunology, 2006, 18(5):0-275. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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