Defined Milestones
Six stages from sequence review to delivery
Custom Protein Expression & Production
A practical route from sequence to application-ready protein
Request NowOverview
PROVETOP provides integrated protein expression and production services for universities, research institutes, biopharmaceutical companies and diagnostic developers. Projects can begin with a sequence, accession number or target name.
We evaluate molecular weight, domain boundaries, transmembrane regions, signal peptides, disulfide bonds, post-translational modifications and downstream use before recommending a construct, host, tag, purification route and quality package.
Available routes include E. coli, mammalian and insect-cell expression, with other systems evaluated when required. Project records and milestone communication are included throughout the service.
Highlights
Flexible Starting Point
Target name, accession or complete sequence
Multiple Platforms
E. coli, mammalian and insect-cell routes
Tailored Solution
Construct, host, tag, buffer and QC by use
Protein Expression Workflow
- Sequence & Construct ReviewTarget analysis and route proposal
- Gene & Vector ConstructionGene synthesis and vector confirmation
- Small-scale Expression ScreeningHost and condition evaluation
- Scale-up Culture & HarvestProduction and sample recovery
- Purification & Buffer ExchangePurification and formulation
- QC, Documentation & DeliveryAgreed testing and project records
Service Details
| Milestones | Specifications | Deliverables | Indicative Time |
|---|---|---|---|
| Sequence Analysis & Plan |
| Feasibility feedback and recommended project route | 1–3 business days |
| Gene & Vector Construction |
| Sequence-confirmed expression construct | 2–3 weeks |
| Expression Evaluation |
| Small-scale screening summary and scale-up recommendation | 1–3 weeks |
| Scale-up & Purification |
| Purified protein sample and buffer record | 1–3 weeks |
| QC & Delivery |
| Sample, agreed QC report, project records and handling guidance | 3–7 business days |
| Turnaround Time | Typical cycle: 3–8 weeks. Difficult proteins and assay development are evaluated individually. | ||
Custom requirements may extend the turnaround time. Final timing is confirmed after technical evaluation.
Case Studies
Case 1: Mammalian expression of a complex glycoprotein
Project need
A human receptor extracellular domain for antibody screening and interaction studies, with multiple glycosylation sites and disulfide bonds.
Technical approach
Domain-boundary and secretion-construct design, mammalian transient expression, affinity purification and SEC polishing.
Project result
A high-purity, homogeneous target protein suitable for downstream antibody binding and molecular-interaction studies.
Case 2: Low-solubility antigen in E. coli
Project need
A low-yield antigen produced mainly as inclusion bodies, requiring a sample suitable for animal immunization.
Technical approach
Adjust expression boundaries and tag position, screen strain, induction temperature and time, then compare soluble-expression and refolding routes.
Project result
Improved soluble expression and a purified sample suitable for the agreed downstream immunization work.
Case 3: Cytokine expression with activity requirements
Project need
A recombinant cytokine with cellular activity for cell culture and in-vitro functional studies, with endotoxin control.
Technical approach
Secreted eukaryotic expression, multi-step purification, aggregation and endotoxin control, followed by a cell-based activity assay.
Project result
A sample meeting the agreed purity, endotoxin and biological-activity requirements for downstream cell experiments.
Quotation and Ordering
Share the protein target, intended use, quantity and quality requirements so we can prepare a project route and quotation.
Submit the target name, sequence and intended application
Our technical team reviews feasibility and system options
You review and approve the route, tests and price
We confirm the schedule and begin the project
Milestone updates, report and sample delivery
Request a Quote
Emailorder@provetop.com
Phone400-829-0116
Online FormCustom protein inquiry
LocationSuzhou Industrial Park