Non-biotinylated Cynomolgus APRIL/TNFSF13 Trimer Protein
Catalog No: APRIL-CP001
- Species
- Cynomolgus
- Expression System
- HEK293
- Tag
- His, Flag, Avi
- Activity
- Activity verified
Product overview
Recombinant Cynomolgus APRIL/TNFSF13 Trimer Protein is expressed in HEK293 cells with a His tag and Flag tag and Avi tag at the N-terminus. It contains amino acid residues Lys112-Leu250 trimer design (UniProt accession: A0A2K5TJA1).
Product Details
- Molecular Aliases
- CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2; 2310026N09Rik
- Protein Length
- Lys112-Leu250
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 52.1 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, 200 mM L-arginine (pH 8.0). Normally 8% mannitol is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Cynomolgus APRIL Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus APRIL Trimer is greater than 95% as determined by SEC-HPLC.
Immobilized Cynomolgus APRIL (Trimer), His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque BCMA, hFc Tag with the EC50 of 2.4 ng/ml determined by ELISA (QC Test).
Immobilized Cynomolgus APRIL Trimer, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-APRIL Antibody, hFc Tag with the EC50 of 13.6ng/ml determined by ELISA.
Loaded Cynomolgus APRIL (Trimer), His Tag on Anti-His-Biosensor can bind Human TACI, hFc Tag with an affinity constant of 4.06 nM as determined in BLI assay.
Loaded Cynomolgus APRIL (Trimer), His-Avi tag on Anti-His-Biosensor can bind Cynomolgus/Rhesus macaque BCMA, hFc Tag with an affinity constant of 1.59 nM as determined in BLI assay.
Background
The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily. Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFFR.
References
- Bossen C, Schneider P. BAFF, APRIL and their receptors: Structure, function and signaling[J]. Seminars in Immunology, 2006, 18(5):0-275.
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