Human TIGIT Protein, Ultra Low Endotoxin
Catalog No: TIGIT-HP002
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Human TIGIT Protein is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met22-Pro141 (UniProt accession: Q495A1-1).
Product Details
- Molecular Aliases
- TIGIT; VSIG9; VSTM3; WUCAM
- Protein Length
- Met22-Pro141
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 14 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.01 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human TIGIT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TIGIT is greater than 95% as determined by SEC-HPLC.
Immobilized Human TIGIT, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD155, hFc Tag with the EC50 of 0.38μg/ml determined by ELISA (QC Test).
Immobilized Human TIGIT, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-TIGIT Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA.
Serial dilutions of Anti-TIGIT Antibody were added into Human TIGIT, His Tag: Biotinylated CD155, hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.46μg/ml.
Human CD155, hFc Tag captured on Protein A chip, can bind Human TIGIT, His Tag with an affinity constant of 0.17μM as determined in a SPR assay (Biacore T200).
Background
TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.
References
- Yu X, Harden K, C Gonzalez L, et al. The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells[J]. NATURE IMMUNOLOGY, 2008, 10(1):48-57. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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