Cynomolgus/Rhesus macaque TIGIT Protein
Catalog No: TIGIT-CP001
- Species
- Cyno/Rhesus
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Cynomolgus/Rhesus macaque TIGIT Protein is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met22-Ile141 (accession: XP_015300911.1).
Product Details
- Molecular Aliases
- TIGIT; VSIG9; VSTM3; WUCAM
- Protein Length
- Met22-Ile141
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 14.4 kDa. Due to glycosylation, the protein migrates to 18-25 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Cynomolgus/Rhesus macaque TIGIT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus/Rhesus macaque TIGIT is greater than 90% as determined by SEC-HPLC.
Immobilized Cynomolgus TIGIT, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human CD155, hFc Tag (Cat. CD1-HM255) with the EC50 of 0.44μg/ml determined by ELISA. (QC Test)
Immobilized Cynomolgus/Rhesus macaque TIGIT, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-TIGIT Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA.
Background
TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.
References
- Yu X, Harden K, C Gonzalez L, et al. The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells[J]. NATURE IMMUNOLOGY, 2008, 10(1):48-57. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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