Mouse TIGIT Protein
Catalog No: TIGIT-MP001
- Species
- Mouse
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Mouse TIGIT Protein is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Gly26-Thr143 (accession: NP_001139797.1).
Product Details
- Molecular Aliases
- TIGIT; VSIG9; VSTM3; WUCAM
- Protein Length
- Gly26-Thr143
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 14 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Mouse TIGIT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse TIGIT is greater than 95% as determined by SEC-HPLC.
Mouse CD155, His Tag immobilized on CM5 Chip can bind Mouse TIGIT, His Tag with an affinity constant of 4.00 μM as determined in SPR assay (Biacore T200).
Background
TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.
References
- Yu X, Harden K, C Gonzalez L, et al. The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells[J]. NATURE IMMUNOLOGY, 2008, 10(1):48-57. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.