Human NY-ESO-1 TCR&CD3 Complex VLP
Catalog No: NYESO1-HP009
- Species
- Human
- Expression System
- HEK293
- Activity
- Activity verified
Product overview
Recombinant Human NY-ESO-1 TCR&CD3 Complex VLP is expressed from HEK293. It contains NY-ESO-1 specific TCR (1G4c113), CD3ε&CD3γ, CD3ε&CD3δ, and CD3ζ dimers
Product Details
- Molecular Aliases
- NY-ESO-1 TCR&CD3
- Expression System
- HEK293
- Purity
- > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS, 300mM L-arginine (pH 7.4). Notice: If you need it for immunization, water-soluble adjuvant is recommended.
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
The purity of Human NY-ESO-1 TCR&CD3 Complex VLP is greater than 95% as determined by SEC-HPLC.
Immobilized Human NY-ESO-1 TCR&CD3 Complex VLP at 5 μg/mL (100μl/well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 18.2ng/ml determined by ELISA. (QC Test)
Immobilized Human NY-ESO-1 TCR&CD3 Complex VLP at 5 μg/mL (100μl/well) on the plate. Dose response curve for Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag (Cat. MHC-HM405) with the EC50 of 7.5ng/ml determined by ELISA.
Background
The 1G4c113 TCR is an engineered, affinity-matured variant of the 1G4 T-cell receptor that specifically recognizes the peptide fragment of NY-ESO-1 (SLLMWITQC) presented on the HLA-A*02:01. Cluster of Differentiation 3 (CD3) is a multimeric protein complex and T cell co-receptor that plays an essential role in adaptive immune responses. These CD3 dimers bind non-covalently to the TCR to form the TCR&CD3 complex on the cell surface.
References
- (1) Zhao, Yangbing, et al. "High-affinity TCRs generated by phage display provide CD4+ T cells with the ability to recognize and kill tumor cell lines." The Journal of Immunology 179.9 (2007): 5845-5854. DOI: 10.4049/jimmunol.179.9.5845 PMCID: PMC2140228, PMID: 17947658 (2) Li, Yi, et al. "Directed evolution of human T-cell receptors with picomolar affinities by phage display." Nature biotechnology 23.3 (2005): 349-354. DOI: 10.1038/nbt1070, PMID: 15723046
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