Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc
Catalog No: NYESO1-HB002
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Biotin
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc is expressed from HEK293. Both the biotin and the His tag are located on the C-terminus of the NY-ESO-1 TCR. It contains NY-ESO-1 specific TCR (1G4c113), CD3ε&CD3γ, CD3ε&CD3δ, and CD3ζ dimers
Product Details
- Molecular Aliases
- NY-ESO-1 TCR&CD3
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 194.8 kDa.
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry.
- State
- Liquid
- Storage Conditions
- Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Immobilized Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 18.3ng/ml determined by ELISA. (QC Test)
Immobilized Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc, His Tag with the EC50 of 0.1μg/ml determined by ELISA.
Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind OKT3, mFc Tag with an affinity constant of 0.20 nM as determined in SPR assay (Biacore T200).
OKT3, mFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc, His Tag with an affinity constant of 0.33 nM as determined in SPR assay (Biacore T200).
Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc captured on CM5 Chip via Streptavidin can bind Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag with an affinity constant of 0.78 nM as determined in SPR assay (Biacore T200).
Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc, His Tag on Streptavidin-Biosensor can bind OKT3, mFc Tag with an affinity constant of 0.89 nM as determined in BLI assay.
Loaded Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc, His Tag on Streptavidin-Biosensor can bind Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag with an affinity constant of 2.37 nM as determined in BLI assay.
Background
The 1G4c113 TCR is an engineered, affinity-matured variant of the 1G4 T-cell receptor that specifically recognizes the peptide fragment of NY-ESO-1 (SLLMWITQC) presented on the HLA-A*02:01. Cluster of Differentiation 3 (CD3) is a multimeric protein complex and T cell co-receptor that plays an essential role in adaptive immune responses. These CD3 dimers bind non-covalently to the TCR to form the TCR&CD3 complex on the cell surface.
References
- (1) Zhao, Yangbing, et al. "High-affinity TCRs generated by phage display provide CD4+ T cells with the ability to recognize and kill tumor cell lines." The Journal of Immunology 179.9 (2007): 5845-5854. DOI: 10.4049/jimmunol.179.9.5845 PMCID: PMC2140228, PMID: 17947658 (2) Li, Yi, et al. "Directed evolution of human T-cell receptors with picomolar affinities by phage display." Nature biotechnology 23.3 (2005): 349-354. DOI: 10.1038/nbt1070, PMID: 15723046
Have questions about this product?
Our technical team can help with product selection, application questions and order support.