Human HLA-A*02:01&B2M&KKLC1 (ILNNFPHSI) Tetramer Protein
Catalog No: CT83-HP004
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Human HLA-A*02:01&B2M&KKLC1 (ILNNFPHSI) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ILNNFPHSI peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &ILNNFPHSI).
Product Details
- Molecular Aliases
- KK-LC-1; CT83; CXorf61; KKLC1;
- Protein Length
- Gly25-Thr305
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Human HLA-A*02:01&B2M&KKLC1 (ILNNFPHSI) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%.
The purity of Human HLA-A*02:01&B2M&KKLC1 (ILNNFPHSI) Tetramer is greater than 95% as determined by SEC-HPLC.
Background
Kita-Kyushu lung cancer antigen-1 (KK-LC-1), encoded by CT83, includes epitope peptides recognized by cytotoxic T lymphocytes (CTLs). CTLs against KK-LC-1 predominantly accumulate among tumor-infiltrating lymphocytes, which leads to a good response to adaptive immunotherapy. Except for the testis, KK-LC-1 is not expressed in normal tissues. It is expressed in 33%, 82%, and 75% of non-small cell lung cancers, GC, and triple-negative breast cancers, respectively.
References
- Otsuka T, Fukuyama T, Futawatari N, Tahara K, Watanabe M, Ichiki Y, Soeno T, Takahashi Y, Yamazaki H, Fujimori Y, Ohshiro T, Kobayashi N, Kida M, Koizumi W, Kusano C. Detection of Kita-Kyushu Lung Cancer Antigen-1, a Cancer/Testis Antigen, in the Stomach Close to a Cancerous Condition. J Cancer. 2022 Oct 31;13(14):3526-3532. doi: 10.7150/jca.67534. PMID: 36484013; PMCID: PMC9723985.
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