Biotinylated Human HLA-A*01:01&B2M&CT83 (NTDNNLAVY) Monomer Protein
Catalog No: CT83-HB002
- Species
- Human
- Expression System
- E. coli
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Human HLA-A*01:01&B2M&CT83 (NTDNNLAVY) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*01:01), Ile21-Met119(B2M) and NTDNNLAVY peptide (accession: Q5SUL5(HLA-A*01:01) & P61769(B2M) &NTDNNLAVY).
Product Details
- Molecular Aliases
- CT83; HLA class I; MHC class I; HLA-A; HLA-A*0101; MHC; MHC HLA-A alpha; NTDNNLAVY
- Protein Length
- Gly25-Thr305
- Expression System
- E. coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 35.84 kDa (HLA-A*01:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Biotinylated Human HLA-A*01:01&B2M&CT83 (NTDNNLAVY) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*01:01&B2M&CT83 (NTDNNLAVY) Monomer is greater than 95% as determined by SEC-HPLC.
Background
Cancer/testis antigens 83 (CT83), also called KK-LC-1 or CXorf61, recognized by cytotoxic T lymphocytes (CTL), has become a promising target for immunotherapy.
References
- Ye Z, Liang Y, Ma Y, Lin B, Cao L, Wang B, Zhang Z, Yu H, Li J, Huang M, Zhou K, Zhang Q, Liu X, Zeng J. Targeted photodynamic therapy of cancer using a novel gallium (III) tris (ethoxycarbonyl) corrole conjugated-mAb directed against cancer/testis antigens 83. Cancer Med. 2018 Jun 1;7(7):3057–65. doi: 10.1002/cam4.1601. Epub ahead of print. PMID: 29856138; PMCID: PMC6051178.
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