Human HLA-A*01:01&B2M&CT83 (NTDNNLAVY) Tetramer Protein
Catalog No: CT83-HP002
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Human CT83(HLA-A*01:01) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus,tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*01:01), Ile21-Met119(B2M) and NTDNNLAVY peptide (accession: Q5SUL5(HLA-A*01:01) & P61769(B2M) &NTDNNLAVY).
Product Details
- Molecular Aliases
- CT83; HLA class I; MHC class I; HLA-A; HLA-A*0101; MHC; MHC HLA-A alpha
- Protein Length
- Gly25-Thr305
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human HLA-A*01:01&B2M&CT83 (NTDNNLAVY) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%.
The purity of Human HLA-A*01:01&B2M&CT83 (NTDNNLAVY) Tetramer is greater than 95% as determined by SEC-HPLC.
Background
Cancer/testis antigens 83 (CT83), also called KK-LC-1 or CXorf61, recognized by cytotoxic T lymphocytes (CTL), has become a promising target for immunotherapy.
References
- Ye Z, Liang Y, Ma Y, Lin B, Cao L, Wang B, Zhang Z, Yu H, Li J, Huang M, Zhou K, Zhang Q, Liu X, Zeng J. Targeted photodynamic therapy of cancer using a novel gallium (III) tris (ethoxycarbonyl) corrole conjugated-mAb directed against cancer/testis antigens 83. Cancer Med. 2018 Jun 1;7(7):3057–65. doi: 10.1002/cam4.1601. Epub ahead of print. PMID: 29856138; PMCID: PMC6051178.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.