Biotinylated Human HLA-A*01:01&B2M&CT83 (NTDNNLAVY) Monomer Protein
Catalog No: CT83-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- with His, Avi
Product overview
Recombinant Biotinylated Human HLA-A*01:01&B2M&CT83 (NTDNNLAVY) Monomer Protein is expressed from HEK293 with with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*01:01), Ile21-Met119(B2M) and NTDNNLAVY peptide (accession: Q5SUL5(HLA-A*01:01) & P61769(B2M) &NTDNNLAVY).
Product Details
- Molecular Aliases
- CT83; HLA class I; MHC class I; HLA-A; HLA-A*0101; MHC; MHC HLA-A alpha; NTDNNLAVY
- Protein Length
- Gly25-Thr305
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 50.30 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human HLA-A*01:01&B2M&CT83 (NTDNNLAVY) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*01:01&B2M&CT83 (NTDNNLAVY) Monomer is greater than 95% as determined by SEC-HPLC.
Background
Cancer/testis antigens 83 (CT83), also called KK-LC-1 or CXorf61, recognized by cytotoxic T lymphocytes (CTL), has become a promising target for immunotherapy.
References
- Ye Z, Liang Y, Ma Y, Lin B, Cao L, Wang B, Zhang Z, Yu H, Li J, Huang M, Zhou K, Zhang Q, Liu X, Zeng J. Targeted photodynamic therapy of cancer using a novel gallium (III) tris (ethoxycarbonyl) corrole conjugated-mAb directed against cancer/testis antigens 83. Cancer Med. 2018 Jun 1;7(7):3057–65. doi: 10.1002/cam4.1601. Epub ahead of print. PMID: 29856138; PMCID: PMC6051178.
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