Human FGF-7/KGF Protein
Catalog No: FGF7-HP001
- Species
- Human
- Expression System
- E. coli
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Human FGF-7/KGF Protein is expressed in E. coli with a His tag at the N-terminus. It contains amino acid residues Cys32-Thr194 (UniProt accession: P21781-1).
Product Details
- Molecular Aliases
- FGF-7; HBGF-7; KGF; FGF7
- Protein Length
- Cys32-Thr194
- Expression System
- E. coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 20.1 kDa same as Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22μm filtered solution in 50mM Tris, 250mM NaCl (pH 7.5).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Human FGF-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human FGF-7 is greater than 95% as determined by SEC-HPLC.
Immobilized Human FGF-7, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human FGFR2 beta (IIIb), hFc Tag with the EC50 of 1.22μg/ml determined by ELISA (QC Test).
Human FGF-7, His Tag immobilized on CM5 Chip can bind Human FGFR2 beta (IIIb), His Tag with an affinity constant of 18.91 nM as determined in SPR assay (Biacore T200).
Background
The expression patterns of mRNAs encoding Fibroblast Growth Factor-7 (FGF-7) and its high affinity receptor suggested that FGF-7 signaling may play a role in regulating ureteric bud growth.t Results of these studies demonstrate that the developing ureteric bud and mature collecting system of FGF-7-null kidneys is markedly smaller than wild type. FGF-7 levels modulate the extent of ureteric bud growth during development and the number of nephrons that eventually form in the kidney.
References
- Qiao J, Uzzo R, Obara-Ishihara T, Degenstein L, Fuchs E, Herzlinger D. FGF-7 modulates ureteric bud growth and nephron number in the developing kidney. Development. 1999 Feb;126(3):547-54. PMID: 9876183.
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