Biotinylated Human FGF-7/KGF Protein
Catalog No: FGF7-HB001
- Species
- Human
- Expression System
- E.coli
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human FGF-7/KGF Protein is expressed in E. coli with a His tag and Avi tag at the N-terminus. It contains amino acid residues Cys32-Thr194 (UniProt accession: P21781-1).
Product Details
- Molecular Aliases
- FGF-7; HBGF-7; KGF;FGF7; HBGF7;Fibroblast growth factor-7; Fibroblast growth factor 7
- Protein Length
- Cys32-Thr194
- Expression System
- E.coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 21.79 kDa. The protein migrates to 25-28 kDa based on Tris-Bis PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human FGF-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human FGFR2 beta (IIIb), hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human FGF-7, His-Avi tag with the EC50 of 26.7ng/ml determined by ELISA (QC Test).
Background
The expression patterns of mRNAs encoding Fibroblast Growth Factor-7 (FGF-7) and its high affinity receptor suggested that FGF-7 signaling may play a role in regulating ureteric bud growth.t Results of these studies demonstrate that the developing ureteric bud and mature collecting system of FGF-7-null kidneys is markedly smaller than wild type. FGF-7 levels modulate the extent of ureteric bud growth during development and the number of nephrons that eventually form in the kidney.
References
- Qiao J, Uzzo R, Obara-Ishihara T, Degenstein L, Fuchs E, Herzlinger D. FGF-7 modulates ureteric bud growth and nephron number in the developing kidney. Development. 1999 Feb;126(3):547-54. PMID: 9876183.
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