Human/Cynomolgus/Rhesus macaque CD40 Ligand/TNFSF5 Trimer Protein
Catalog No: CD40-HF002
- Species
- Human/Cyno/Rhesus
- Expression System
- HEK293
- Tag
- monomeric hFc
- Activity
- Activity verified
Product overview
Recombinant Human/Cynomolgus/Rhesus macaque CD40 Ligand/TNFSF5 Trimer Protein is expressed in HEK293 cells with monomeric hFc tag at the N-terminus. It contains amino acid residues Met113-Leu261 trimer design (accession: P29965(Human) / G7Q1T4(Cynomolgus) / P63304(Rhesus macaque)).
Product Details
- Molecular Aliases
- CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand
- Protein Length
- Met113-Leu261
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 74.9 kDa. Due to glycosylation, the protein migrates to 75-80 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human/Cynomolgus/Rhesus macaque CD40 Ligand (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human/Cynomolgus/Rhesus macaque CD40 Ligand (Trimer) is greater than 95% as determined by SEC-HPLC.
Immobilized Human CD40, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human/Cynomolgus/Rhesus macaque CD40 Ligand (Trimer), hFc Tag with the EC50 of 29.8ng/ml determined by ELISA (QC Test).
Serial dilutions of Anti-CD40 Antibody were added into Biotinylated Human CD40, His Tag: Human/Cynomolgus/Rhesus macaque CD40 Ligand (Trimer), hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 2.0ug/ml.
Loaded Human CD40, His Tag on Anti-His-Biosensor can bind Human CD40 Ligand (Trimer), monomeric hFc Tag with an affinity constant of 0.61 nM as determined in BLI assay.
Background
CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells..
References
- Bjorck P, Filbert E, Zhang Y, et al. The CD40 agonistic monoclonal antibody APX005M has potent immune stimulatory capabilities[J]. Journal for Immunotherapy of Cancer, 2015, 3(S2):P198.
- Pound, J. D. Minimal cross-linking and epitope requirements for CD40-dependent suppression of apoptosis contrast with those for promotion of the cell cycle and homotypic adhesions in human B cells[J]. International Immunology, 1999, 11(1):11-20 Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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