Biotinylated Human/Cynomolgus/Rhesus macaque CD40 Ligand/TNFSF5 Trimer Protein (Primary Amine Labeling)
Catalog No: CD40-HB004
- Species
- Human/Cyno/Rhesus
- Expression System
- HEK293
- Tag
- His, Flag
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human/Cynomolgus/Rhesus macaque CD40 Ligand/TNFSF5 Trimer Protein (Primary Amine Labeling) is expressed in HEK293 cells with a His tag and Flag tag at the N-terminus. It contains amino acid residues Met113-Leu261 trimer design (accession: P29965(Human) / G7Q1T4(Cynomolgus) / P63304(Rhesus macaque)).
Product Details
- Molecular Aliases
- CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand
- Protein Length
- Met113-Leu261
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 52.4 kDa, Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer is greater than 95% as determined by SEC-HPLC.
Immobilized Human CD40, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer, His Tag with the EC50 of 47.6ng/ml determined by ELISA (QC Test).
Background
CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.
References
- Bjorck P, Filbert E, Zhang Y, et al. The CD40 agonistic monoclonal antibody APX005M has potent immune stimulatory capabilities[J]. Journal for Immunotherapy of Cancer, 2015, 3(S2): P198.
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