Mouse CD40 Ligand/TNFSF5 Trimer Protein
Catalog No: CD40-MF001
- Species
- Mouse
- Expression System
- HEK293
- Tag
- monomeric hFc
- Activity
- Activity verified
Product overview
Recombinant Mouse CD40 Ligand/TNFSF5 Trimer Protein is expressed in HEK293 cells with monomeric hFc tag at the N-terminus. It contains amino acid residues Met112-Leu260 trimer design (UniProt accession: P27548).
Product Details
- Molecular Aliases
- CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand
- Protein Length
- Met112-Leu260
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 75.6 kDa. Due to glycosylation, the protein migrates to 76-80 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS, 200 mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Mouse CD40 Ligand Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse CD40 Ligand Trimer is greater than 95% as determined by SEC-HPLC.
Immobilized Mouse CD40, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA. (QC Test)
Loaded Mouse CD40, His Tag on Anti-His-Biosensor can bind Mouse CD40 Ligand (Trimer), monomeric hFc Tag with an affinity constant of 0.10 nM as determined in BLI assay.
Background
CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.
References
- Pound, J. D. Minimal cross-linking and epitope requirements for CD40-dependent suppression of apoptosis contrast with those for promotion of the cell cycle and homotypic adhesions in human B cells[J]. International Immunology, 1999, 11(1):11-20 Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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