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Biotinylated Human HLA-A*02:01&B2M&KKLC1 (ILNNFPHSI) Monomer Protein

Catalog No: CT83-HB003

Species
Human
Expression System
HEK293
Tag
His, Avi

Product overview

Recombinant Biotinylated Human HLA-A*02:01&B2M&KKLC1 (ILNNFPHSI) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and ILNNFPHSI peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &ILNNFPHSI).

Product Details

Molecular Aliases
KKLC1; KK-LC-1; CT83; CXorf61
Protein Length
Gly25-Thr305
Expression System
HEK293
Theoretical Molecular Weight
The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Bis-Tris PAGE result.
Purity
> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin
Less than 1 EU per μg by the LAL method.
Buffer / Formulation
Supplied as 0.22 μm filtered solution in PBS, 100mM L-Arginine (pH 7.4).
State
Liquid
Storage Conditions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data Display

Bis-Tris PAGE
CT83-HB003 Bis-Tris-PAGE result

Biotinylated Human HLA-A*02:01&B2M&KKLC1 (ILNNFPHSI) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC
CT83-HB003 SEC-HPLC result

The purity of Biotinylated Human HLA-A*02:01&B2M&KKLC1 (ILNNFPHSI) Monomer is greater than 95% as determined by SEC-HPLC.

Background

ILNNFPHSI is a peptide epitope derived from KKLC1 (also known as Kita-kyushu lung cancer antigen 1, CT83 or CXorf61) that is naturally processed and presented on the cell surface in complex with MHC class I molecules. This peptide, together with binding moieties that specifically recognize the peptide–MHC complex, provides a valuable basis for the development of therapeutic agents capable of selectively targeting KKLC1-expressing cancer cells, and holds particular promise for the treatment of malignancies such as non-small cell lung cancer (adenocarcinoma and squamous subtypes) and esophageal cancer.

References

  1. [1] Fukuyama T, Hanagiri T, Takenoyama M, Ichiki Y, Mizukami M, So T, Sugaya M, So T, Sugio K, Yasumoto K. Identification of a new cancer/germline gene, KK-LC-1, encoding an antigen recognized by autologous CTL induced on human lung adenocarcinoma. Cancer Res. 2006 May 1;66(9):4922-8. doi: 10.1158/0008-5472.CAN-05-3840. PMID: 16651449.
  2. [2] Paret C, Simon P, Vormbrock K, Bender C, Kölsch A, Breitkreuz A, Yildiz Ö, Omokoko T, Hubich-Rau S, Hartmann C, Häcker S, Wagner M, Roldan DB, Selmi A, Türeci Ö, Sahin U. CXorf61 is a target for T cell based immunotherapy of triple-negative breast cancer. Oncotarget. 2015 Sep 22;6(28):25356-67. doi: 10.18632/oncotarget.4516. PMID: 26327325; PMCID: PMC4694836.

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