Human FGF10 Protein
Catalog No: FGF10-HP001
- Species
- Human
- Expression System
- E. coli
- Activity
- Activity verified
Product overview
Recombinant Human FGF10 Protein is expressed in E. coli without tag. It contains amino acid residues Gln38-Ser208 (UniProt accession: O15520).
Product Details
- Molecular Aliases
- FGF-10; KGF2
- Protein Length
- Gln38-Ser208
- Expression System
- E. coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 19.3 kDa. The protein migrates to 25 kDa based on Bis-Tris PAGE result.
- Purity
- > 90% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human FGF10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Human FGF10, No Tag immobilized on CM5 Chip can bind Human FGFR2 beta (IIIb), hFc Tag with an affinity constant of 8.43 nM as determined in SPR assay (Biacore T200).
Background
Fibroblast growth factor 10 (FGF10) regulates multiple stages of structural lung morphogenesis, cellular differentiation, and the response to injury. As a driver of lung airway branching morphogenesis, FGF10 signaling defects during development lead to neonatal lung disease. Lung diseases impact patients across the lifespan, from infants in the first minutes of life through the aged population. Congenital abnormalities of lung structure can cause lung disease at birth or make adults more susceptible to chronic disease.
References
- Prince LS. FGF10 and Human Lung Disease Across the Life Spectrum. Front Genet. 2018 Oct 31;9:517. doi: 10.3389/fgene.2018.00517. PMID: 30429870; PMCID: PMC6220039.
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