GMP Human IL-15
Catalog No: IL15-HP002
- Species
- Human
- Expression System
- E. coli
- Activity
- Activity verified
Product overview
Recombinant GMP Human IL-15 is expressed in E. coli without tag. It contains amino acid residues Asn49-Ser162 (UniProt accession: P40933-1).
Product Details
- Molecular Aliases
- Interleukin-15; IL-15; IL15; il 15; MGC9721
- Protein Length
- Asn49-Ser162
- Expression System
- E. coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 12.8 kDa same as Bis-Tris PAGE result.
- Purity
- ≥ 95% as determined by SEC-HPLC.
- Endotoxin
- ≤ 0.01 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- The product is shipped with ice bag. Store it immediately at -20°C±5°C upon arrival. -20°C±5°C for 36 months in lyophilized state. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
GMP Human IL-15 on Bis-Tris PAGE under reduced condition.
Measured in a cell proliferation assay using CTLL-2 cells. The ED50 for this effect is 0.1 - 0.3 ng/mL. The specific activity of GMP Human IL-15 is > 1 × 10^7 IU/mg, which is calibrated against the human IL-15 WHO reference standard (NIBSC code: 95/554) (QC Test).
GMP Human IL-15 (reconstituted protein) was stored at 37°C, the protein is stable for 7 days determined by cell based assay.
GMP Human IL-15 (reconstituted protein) was stored at 4°C, the protein is stable for 90 days determined by cell based assay.
GMP Human IL-15 (reconstituted protein) was repeatedly frozen at -80°C and thawed at 37°C, the protein is stable after freezing and thawing 3 cycles determined by cell based assay.
Background
IL-15 belongs to the common gamma chain cytokine family and has pleiotropic immunological functions, and the protein is mainly produced by monocytes, dendritic cells, and epithelial cells. IL-15 plays a crucial role in the development, homeostasis, and function of T, NK and NK-T cells, and is additionally required for various functions of the B cells, dendritic cells (DC), macrophages, and mast cells. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA.
References
- Lee H, Park SH, Shin EC. IL-15 in T-Cell Responses and Immunopathogenesis. Immune Netw. 2024 Feb 16;24(1):e11.
- Patidar M, Yadav N, Dalai SK. Interleukin 15: A key cytokine for immunotherapy. Cytokine Growth Factor Rev. 2016 Oct;31:49-59.
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