Biotinylated Human IL-15RA&IL-15 Protein (Primary Amine Labeling)
Catalog No: IL15-HB003
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human IL-15RA&IL-15 Protein (Primary Amine Labeling) is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Ile31-Ser108 (IL-15RA)&Asn49-Ser162 (IL-15) (accession: Q13261-1 (IL-15RA) & P40933-1 (IL-15)).
Product Details
- Molecular Aliases
- IL-15 R alpha; CD215; IL15RA; IL-15RA; IL-15R-alpha1; Interleukin-15; IL-15; IL15; Nanrilkefusp alfa; IL15R alpha; IL15R-alpha1; IL 15; Interleukin 15
- Protein Length
- Ile31-Ser108
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 23.8 kDa. Due to glycosylation, the protein migrates to 25-28 kDa&30-35 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per ug by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human IL-15RA&IL-15 (Primary Amine Labeling) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Biotinylated Human IL-15Ra&IL-15, His Tag at 1 μg/ml (100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Human IL-2 R beta, hFc Tag with the EC50 of 7.8 ng/ml determined by ELISA.
Background
Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development.
References
- Nadeau L, Aguer C. Interleukin-15 as a myokine: mechanistic insight into its effect on skeletal muscle metabolism. Appl Physiol Nutr Metab. 2019 Mar;44(3):229-238. doi: 10.1139/apnm-2018-0022. Epub 2018 Sep 6. PMID: 30189147.
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