Rat TIGIT Protein, Ultra Low Endotoxin
Catalog No: TIGIT-RP002
- Species
- Rat
- Expression System
- HEK293
- Tag
- an mFc (IgG2a)
- Activity
- Activity verified
Product overview
Recombinant Rat TIGIT Protein is expressed in HEK293 cells with an mFc (IgG2a) tag at the C-terminus. It contains amino acid residues Ala17-Gln136 (accession: D3ZTQ2).
Product Details
- Molecular Aliases
- TIGIT; VSIG9; VSTM3; WUCAM
- Protein Length
- Ala17-Gln136
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 39.47 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.01 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Rat TIGIT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Rat TIGIT is greater than 95% as determined by SEC-HPLC.
Immobilized Mouse CD155, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Rat TIGIT, mFc Tag with the EC50 of 23.1ng/ml determined by ELISA. (QC Test)
Loaded Mouse CD155, His Tag on Anti-His-Biosensor can bind Rat TIGIT, mFc Tag with an affinity constant of 6.93 nM as determined in BLI assay.
Background
TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.
References
- Yu X, Harden K, C Gonzalez L, et al. The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells[J]. NATURE IMMUNOLOGY, 2008, 10(1):48-57. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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