Mouse SLAMF1/SLAM/CD150 Protein, Ultra Low Endotoxin
Catalog No: SLAMF1-MP002
- Species
- Mouse
- Expression System
- HEK293
- Tag
- His
Product overview
Recombinant Mouse SLAMF1/SLAM/CD150 Protein is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Thr25-Pro242 (UniProt accession: Q9QUM4-1).
Product Details
- Molecular Aliases
- CDw150; IPO-3; CD150; SLAMF1; SLAM
- Protein Length
- Thr25-Pro242
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 25.3 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.01 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Mouse SLAMF1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse SLAMF1 is greater than 95% as determined by SEC-HPLC.
Background
The signaling lymphocytic activation molecule (SLAM) family is comprised of nine distinct receptors (SLAMF1 through SLAMF9) that are expressed on hematopoietic cells. The SLAM family receptor function is largely controlled via SLAM associated protein (SAP) family adaptors. The SAP family adaptors consist of SAP, Ewing sarcoma associated transcript (EAT)-2, and EAT-2-related transducer (ERT).
References
- Ma C, Wang N, Detre C, Wang G, O'Keeffe M, Terhorst C. Receptor signaling lymphocyte-activation molecule family 1 (Slamf1) regulates membrane fusion and NADPH oxidase 2 (NOX2) activity by recruiting a Beclin-1/Vps34/ultraviolet radiation resistance-associated gene (UVRAG) complex. J Biol Chem. 2012 May 25;287(22):18359-65. doi: 10.1074/jbc.M112.367060. Epub 2012 Apr
- PMID: 22493499; PMCID: PMC3365751. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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