Biotinylated Human SLAMF1/SLAM/CD150 Protein
Catalog No: SLAMF1-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Human SLAMF1/SLAM/CD150 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ala21-Pro237 (UniProt accession: Q13291-1).
Product Details
- Molecular Aliases
- CDw150; IPO-3; CD150; SLAMF1; SLAM
- Protein Length
- Ala21-Pro237
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 27.2 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human SLAMF1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human SLAMF1 is greater than 95% as determined by SEC-HPLC.
Background
SLAMF1/CD150 receptor is a founder of signaling lymphocyte activation molecule (SLAM) family of cell-surface receptors. It is widely expressed on cells within hematopoietic system. In hematologic malignancies CD150 cell surface expression is restricted to cutaneous T-cell lymphomas, few types of B-cell non-Hodgkin's lymphoma, near half of cases of chronic lymphocytic leukemia, Hodgkin's lymphoma, and multiple myeloma.
References
- Gordiienko I, Shlapatska L, Kovalevska L, Sidorenko SP. SLAMF1/CD150 in hematologic malignancies: Silent marker or active player? Clin Immunol. 2019 Jul;204:14-22. doi: 10.1016/j.clim.2018.10.015. Epub 2018 Oct
- PMID:
- Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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