Mouse Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer Protein
Catalog No: QA1B-HP001
- Species
- Mouse
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Mouse Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains His23-Pro296(Qa-1b), Ile21-Met119(B2M) and AMAPRTLLL peptide (accession: P06339(Q1-1b) & P01887(B2M) &AMAPRTLLL).
Product Details
- Molecular Aliases
- Qdm; Qa-1b; MHC; H2-T23
- Protein Length
- His23-Pro296
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 50.60 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Mouse Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer is greater than 95% as determined by SEC-HPLC.
Background
Qa-1b binds a peptide (AMAPRTLLL), referred to as Qdm (for Qa-1 determinant modifier), derived from the signal sequence of murine class Ia molecules. This peptide binds with high affinity and accounts for almost all of the peptides associated with this molecule. Human histocompatibility leukocyte antigen (HLA)-E, a homologue of Qa-1b, binds similar peptides derived from human class Ia molecules and interacts with CD94/NKG2 receptors on natural killer cells.
References
- Kurepa Z, Hasemann CA, Forman J. Qa-1b binds conserved class I leader peptides derived from several mammalian species. J Exp Med. 1998 Sep 7;188(5):973-8. doi: 10.1084/jem.188.5.973. PMID: 9730898; PMCID: PMC2213384.
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