Mouse IgG1 Fc Protein
Catalog No: IGG1FC-MP001
- Species
- Mouse
- Expression System
- HEK293
- Tag
- Fc
- Activity
- Activity verified
Product overview
Recombinant Mouse IgG1 Fc Protein is expressed in HEK293 cells without tag. It contains amino acid residues Val98-Lys324 (UniProt accession: P01868).
Product Details
- Molecular Aliases
- IgG1; Igh-4; RGD1359539; VH7183
- Protein Length
- Val98-Lys324
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 25.7 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Mouse IgG1 Fc on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse IgG1 Fc is greater than 90% as determined by SEC-HPLC.
Mouse FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse IgG1 Fc, No Tag with an affinity constant of 15.09 nM as determined in SPR assay (Biacore T200).
Background
Recombinant murine Fc fragment contains the hinge region, CH2, and CH3 region of the mouse IgG1 isotype. IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues.
References
- Sockolosky J T, Szoka F C. The neonatal Fc receptor, FcRn, as a target for drug delivery and therapy[J]. Advanced Drug Delivery Reviews, 2015:S0169409X15000162.
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