Human IgG1 Fc Protein, Ultra Low Endotoxin
Catalog No: IGG1FC-HP002
- Species
- Human
- Expression System
- HEK293
- Tag
- Fc
- Activity
- Activity verified
Product overview
Recombinant Human IgG1 Fc is expressed in HEK293 cells without tag. It contains amino acid residues Asp104-Lys330 (UniProt accession: P01857-1).
Product Details
- Molecular Aliases
- IgG1; Igh-4; RGD1359539; VH7183
- Protein Length
- Asp104-Lys330
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 25.5 kDa. Due to glycosylation, the protein migrates to 31-33 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.01 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human IgG1 Fc on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IgG1 Fc is greater than 95% as determined by SEC-HPLC.
Loaded Biotinylated Human FcRn, His-Avi tag on Streptavidin-Biosensor can bind Human IgG1 Fc, No Tag with an affinity constant of 22.8 nM as determined in BLI assay. (QC Test)
Human FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Human IgG1 Fc, No Tag with an affinity constant of 0.28 μM as determined in SPR assay (Biacore T200).
Background
IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues.IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.
References
- Ponstingl H, Hilschmann N. [Rule of antibody structure. The primary structure of a monoclonal IgG1 immunoglobulin (myeloma protein Nie), II: isolation and amino acid sequence of the tyrptic peptides from the H-chain (author's transl)].[J]. Hoppe Seylers Zeitschrift Für Physiologische Chemie, 1976, 357(11):1541.
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