Mouse ICOS/CD278 Protein
Catalog No: CD278-MF001
- Species
- Mouse
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Mouse ICOS/CD278 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Glu21-Leu144 (UniProt accession: Q9WVS0).
Product Details
- Molecular Aliases
- AILIM; CD278; CRP-1; CVID1; ICOS; MGC39850;CRP1
- Protein Length
- Glu21-Leu144
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 40.8 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Mouse ICOS on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Mouse B7-H2, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Mouse ICOS, hFc Tag with the EC50 of 91.2ng/ml determined by ELISA. (QC Test)
Mouse ICOS, hFc Tag captured on CM5 Chip via Protein A can bind Mouse B7-H2, His Tag with an affinity constant of 0.329 μM as determined in SPR assay (Biacore T200).
Loaded Mouse ICOS, hFc Tag on ProA-Biosensor can bind Rat B7-H2, His Tag with an affinity constant of 0.40 µM as determined in BLI assay.
Background
Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain.
References
- ICOS Costimulation at the Tumor Site in Combination with CTLA-4 Blockade Therapy Elicits Strong Tumor Immunity. 2019
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