Human ICOS/CD278 (Q50A) Protein
Catalog No: CD278-HF002
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human ICOS/CD278 (Q50A) Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Glu21-Phe141 (UniProt accession: Q9Y6W8-1).
Product Details
- Molecular Aliases
- AILIM; CD278; CRP-1; CVID1; ICOS; MGC39850;CRP1
- Protein Length
- Glu21-Phe141
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 40.45 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human ICOS (Q50A) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human ICOS (Q50A) is greater than 95% as determined by SEC-HPLC.
Immobilized Human ICOS (Q50A), hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-ICOS Antibody, hFc Avi tag with the EC50 of 91.0ng/ml determined by ELISA. (QC Test)
Human ICOS (Q50A), hFc Tag captured on CM5 Chip via Protein A can bind Human B7-H2, His Tag with an affinity constant of 41.98 nM as determined in SPR assay (Biacore T200).
Loaded Human ICOS (Q50A), hFc Tag on ProA-Biosensor can bind Human B7-H2, His Tag with an affinity constant of 0.32 µM as determined in BLI assay.
Background
Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain.
References
- ICOS Costimulation at the Tumor Site in Combination with CTLA-4 Blockade Therapy Elicits Strong Tumor Immunity. 2019 Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.