Human VCAM-1/CD106 Protein
Catalog No: CD106-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
Product overview
Recombinant Human VCAM-1/CD106 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Phe25-Glu698 (accession: NP_001069).
Product Details
- Molecular Aliases
- V-CAM 1; VCAM-1; INCAM-100; CD106; DKFZp779G2333; MGC99561;VCAM1; INCAM100
- Protein Length
- Phe25-Glu698
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 100.17 kDa. Due to glycosylation, the protein migrates to 105-125 kDa based on Bis-Tris PAGE result.
- Purity
- > 90% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human VCAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
Background
Tumor necrosis factor alpha (TNFα) is a pro-inflammatory cytokine that triggers the expression of inflammatory molecules, including other cytokines and cell adhesion molecules. TNFα induces the expression of intercellular cell adhesion molecule-1 and vascular cell adhesion molecule-1 (VCAM-1). VCAM-1 was originally identified as a cell adhesion molecule that helps regulate inflammation-associated vascular adhesion and the transendothelial migration of leukocytes, such as macrophages and T cells.
References
- Kong DH, Kim YK, Kim MR, Jang JH, Lee S. Emerging Roles of Vascular Cell Adhesion Molecule-1 (VCAM-1) in Immunological Disorders and Cancer. Int J Mol Sci. 2018 Apr 2;19(4):1057. doi: 10.3390/ijms19041057. PMID: 29614819; PMCID: PMC5979609.
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