Human Transferrin R/CD71 Protein
Catalog No: CD71-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human Transferrin R/CD71 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Cys89-Phe760 (UniProt accession: P02786-1).
Product Details
- Molecular Aliases
- TR; TfR1; CD71; sTfR; T9; p90; TFRC; TFR
- Protein Length
- Cys89-Phe760
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 101.8 kDa. Due to glycosylation, the protein migrates to 105-115 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human Transferrin R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Biotinylated Human Transferrin, His Avi tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human Transferrin R, hFc Tag with the EC50 of 58.4ng/ml determined by ELISA. (QC Test)
Immobilized Human Transferrin R, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-Transferrin R Antibody, hFc Tag with the EC50 of 26.3ng/ml determined by ELISA.
Background
The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.
References
- Jhee JH, Nam BY, Park JT, Kim HW, Chang TI, Kang EW, Lim BJ, Yoo TH, Kang SW, Jeong HJ, Han SH. CD71 mesangial IgA1 receptor and the progression of IgA nephropathy. Transl Res. 2021 Apr;230:34-43. doi: 10.1016/j.trsl.2020.10.007. Epub 2020 Oct
- PMID: 33122053.
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