Biotinylated Human Transferrin R/CD71 Protein
Catalog No: CD71-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human Transferrin R/CD71 Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus. It contains amino acid residues Cys89-Phe760 (UniProt accession: P02786).
Product Details
- Molecular Aliases
- TR; TfR1; CD71; sTfR; T9; p90; TFRC; TFR
- Protein Length
- Cys89-Phe760
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 78-95 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 85% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine, 8% trehalose (pH 7.4).
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human Transferrin R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Transferrin R is greater than 85% as determined by SEC-HPLC.
The purity of Biotinylated Human Transferrin R is greater than 85% and the molecular weight of this protein is around 160-180 kDa as determined by SEC-MALS.
Immobilized Human Transferrin, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 66.4ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human Transferrin R, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-Transferrin R Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA.
Human Transferrin, His Tag immobilized on CM5 Chip can bind Biotinylated Human Transferrin R, His-Avi tag with an affinity constant of 7.73 nM as determined in SPR assay (Biacore T200).
Background
The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.
References
- Jhee JH, Nam BY, Park JT, Kim HW, Chang TI, Kang EW, Lim BJ, Yoo TH, Kang SW, Jeong HJ, Han SH. CD71 mesangial IgA1 receptor and the progression of IgA nephropathy. Transl Res. 2021 Apr;230:34-43. doi: 10.1016/j.trsl.2020.10.007. Epub 2020 Oct 24. PMID: 33122053.
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