Human Siglec-15/CD33L3 Protein
Catalog No: CD33L3-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human Siglec-15/CD33L3 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Lys24-Gly260 (UniProt accession: Q6ZMC9-1).
Product Details
- Molecular Aliases
- CD33 molecule-like 3; CD33L3; HsT1361; Siglec15; CD33 antigen-like 3; SIGLEC-15
- Protein Length
- Lys24-Gly260
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 52.6 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in 20mM NaAC (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in 20mM NaAC (pH 5.0). Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human Siglec-15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human Siglec-15, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 22.4ng/ml determined by ELISA.
Background
Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-15 consists of a 244 amino acid extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates.
References
- Jun Wang,et al.Siglec-15 as an immune suppressor and potential target for normalization cancer immunotherapy.Nature Medicine (2019).
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