Human/Rhesus macaque/Mouse/Rat/Canine BMP2 Protein
Catalog No: BMP2-HP001
- Species
- Human
- Expression System
- E. coli
- Activity
- Activity verified
Product overview
Recombinant Human/Rhesus macaque/Mouse/Rat/Canine BMP2 Protein is expressed in E. coli without tag. It contains amino acid residues Gln283-Arg396 (Human/Rhesus macaque) / Gln279-Arg393 (Mouse/Rat) / Gln282-Arg395 (Canine) (accession: P12643(Human) / G7N2Y0(Rhesus macaque) / P21274(Mouse) / P49001(Rat) / A0A8I3PXE9(Canine)).
Product Details
- Molecular Aliases
- BMP-2; BMP-2A; BMP2A; BDA2; SSFSC;
- Protein Length
- Gln283-Arg396
- Expression System
- E. coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 12.90 kDa same as Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 0.5 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in 100mM Acetic Acid (pH 2.8). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in 100mM Acetic Acid (pH 2.8). Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human/Rhesus macaque/Mouse/Rat/Canine BMP2 on Bis-Tris PAGE under Non reduced (N) condition and reduced condition. The purity is greater than 95%.
Measured by its ability to induce Alkaline Phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 100-400 ng/mL. (QC Test)
Background
Bone morphogenetic protein 2 (BMP2), a member of the transforming growth factor-β (TGF-β) super-family, is one of the main chondrogenic growth factors involved in cartilage regeneration. BMP2 is known to induce chondrogenic differentiation in various types of stem cells in vitro. However, BMP2 also induces osteogenic differentiation and endochondral ossification in mesenchymal stem cells (MSCs).
References
- PMID: 27083447.
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