Human Noggin Protein
Catalog No: NOGGIN-HP001
- Species
- Human
- Expression System
- CHO
- Activity
- Activity verified
Product overview
Recombinant Human Noggin Protein is expressed in CHO cells without tag. It contains amino acid residues Gln28-Cys232 (UniProt accession: Q13253).
Product Details
- Molecular Aliases
- Noggin; NOG; SYM1; SYNS1
- Protein Length
- Gln28-Cys232
- Expression System
- CHO
- Theoretical Molecular Weight
- The protein has a predicted MW of 23.05 kDa. Due to glycosylation, the protein migrates to 30-36 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.05 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human Noggin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Noggin is greater than 95% as determined by SEC-HPLC.
Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 4-80 ng/mL in the presence of 50 ng/mL of recombinant Human BMP‑4.
Background
Noggin is an antagonist of bone morphogenetic proteins (BMP), being indispensable for certain developmental events.Noggin expression positively correlated with EGFR expression in both GC cell line models and The Cancer Genome Atlas human GC cohort. Targeting EGFR and its downstream pathways diminished cell proliferation which was promoted by Noggin. Noggin promotes the proliferation of GC cells by upregulating EGFR and enhancing a vicious circle formed by β‑catenin, EGFR, ERK and Akt.
References
- Sun Z, Gao X, Zabkiewicz C, Ruge F, Xie M, Cai S, Sun PH, Griffiths P, Pugh MR, Ji J, Jiang WG, Ye L. Noggin is associated with a poor prognosis of gastric cancer by promoting the proliferation of gastric cancer cells via the upregulation of EGFR. Int J Oncol. 2020 Sep;57(3):813-824. doi: 10.3892/ijo.2020.5081. Epub 2020 Jun
- PMID: 32705152.
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