Human Jagged 1/JAG1 Protein
Catalog No: JAGGED1-HP001
- Species
- Human
- Expression System
- HEK293
- Tag
- His
Product overview
Recombinant Human Jagged 1/JAG1 Protein is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Gln34-Ser1046 (UniProt accession: P78504-1).
Product Details
- Molecular Aliases
- Jagged1; hJ1; CD339; JAGL1; AGS; AHD; AWS
- Protein Length
- Gln34-Ser1046
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 111.56 kDa. Due to glycosylation, the protein migrates to 130-160 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human Jagged 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Jagged 1 is greater than 95% as determined by SEC-HPLC.
Background
JAGGED1 (JAG1) is composed of a relatively small intracellular domain, a transmembrane domain and a larger extracellular component. JAG1 is widely expressed throughout mammalian development, across many tissues and developmental stages. The Notch signaling pathway and JAG1 in particular have been implicated in multiple forms of cancer and multiple aspects of cancer biology including tumor angiogenesis, neoplastic cell growth, cancer stem cell maintenance and the metastatic process.
References
- PMID: 26548814; PMCID: PMC4673022.
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