Human IL-33 Protein
Catalog No: IL33-HP001
- Species
- Human
- Expression System
- E. coli
- Activity
- Activity verified
Product overview
Recombinant Human IL-33 Protein is expressed in E. coli without tag. It contains amino acid residues Ser112-Thr270 (accession: NP_254274.1).
Product Details
- Molecular Aliases
- C9orf26 Z; DVS27; IL1F11; NF-HEV; NFEHEV; IL33; DV27; C9ORF26; RP11-575C20.2
- Protein Length
- Ser112-Thr270
- Expression System
- E. coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 18 kDa same as Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human IL-33 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-33 is greater than 95% as determined by SEC-HPLC.
Immobilized Human IL-33, No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-1RL1, His Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test).
Immobilized Human IL-33, No Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-33 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA.
Immobilized Human IL-33, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-33 Antibody2, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA.
Measured by a reporter gene assay using IL33 Effector Reporter Cell line. The ED50 for this effect is < 0.5 ng/mL.
Human IL-1RL1, hFc Tag (Cat. ST2-HM2L1) captured on CM5 Chip via Protein A can bind Human IL-33, No Tag with an affinity constant of 0.11 nM as determined in SPR assay (Biacore T200).
Background
Interleukin-33 (IL-33) is a cytokine belonging to the IL-1 family, playing a role in inflammatory, infectious and autoimmune diseases and expressed in the cellular nucleus in several tissues. High levels of IL-33 are expressed in epithelial barrier tissues and endothelial barriers. ST2 is a receptor for IL-33, expressed selectively on a subset of Th2 cells, mediating some of their functions.
References
- Liew FY, Girard JP, Turnquist HR. Interleukin-33 in health and disease. Nat Rev Immunol. 2016;16(11):676-689. doi:10.1038/nri.2016.95
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