Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer Protein
Catalog No: MAGEA4-HP004
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and GVYDGREHTV peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &GVYDGREHTV).
Product Details
- Molecular Aliases
- HLA0201; MHC I; MAGE-A4; CT1.4; MAGE4A; MAGE4B; MAGE-X2; member 4
- Protein Length
- Gly25-Thr305
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%.
The purity of Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer is greater than 95% as determined by SEC-HPLC.
Immobilized Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).
Anti-HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer, His Tag with an affinity constant of 8.49pM as determined in SPR assay (Biacore T200).
Background
Melanoma-associated antigen 4 is a protein that in humans is encoded by the MAGEA4 gene. The MAGE- A4 antigen is among the most commonly expressed cancer testis antigens. The Human HLA-A*0201 MAGE-A4 (GVYDGREHTV) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and GVYDGREHTV peptide of the MAGE-A4.
References
- (1)Kageyama S, Ikeda H, Miyahara Y, et al. Adoptive Transfer of MAGE-A4 T-cell Receptor Gene-Transduced Lymphocytes in Patients with Recurrent Esophageal Cancer[J]. Clinical Cancer Research, 2015, 21(10):2268.
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