Human HGF Protein
Catalog No: HGF-HP001
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Human HGF Protein is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Gln32-Ser728 (UniProt accession: P14210-1).
Product Details
- Molecular Aliases
- Hepatopoietin-A; SF; HPTA; HGF; DFNB39; F-TCF; F-TCFB; HGFB
- Protein Length
- Gln32-Ser728
- Expression System
- HEK293
- Theoretical Molecular Weight
- The HGF single chain can be processed into the active form of disulfide-linked heterodimer of α and β chain. The protein has a predicted MW of 79.6 kDa (after cleavage of the signal peptide). Due to glycosylation, the protein migrates to 60-65 kDa and 30-40 kDa corresponding to α chain and β chain based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human HGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human HGF Protein is greater than 95% as determined by SEC-HPLC.
Immobilized Human HGF, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human HGF R, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. (QC Test)
Loaded Human HGF R, hFc Tag on ProA-Biosensor can bind Human HGF, His Tag with an affinity constant of 4.32 nM as determined in BLI assay.
Background
Hepatocyte growth factor (HGF) is an important component of the pathophysiology of IR, with increased levels in most common IR conditions, including obesity. HGF has a role in the metabolic flux of glucose in different insulin sensitive cell types.
References
- Oliveira AG, Araújo TG, Carvalho BM, Rocha GZ, Santos A, Saad MJA. The Role of Hepatocyte Growth Factor (HGF) in Insulin Resistance and Diabetes. Front Endocrinol (Lausanne). 2018 Aug 30;9:503. doi: 10.3389/fendo.2018.00503. PMID: 30214428; PMCID: PMC6125308.
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