Human GITR/TNFRSF18 Protein
Catalog No: GITR-HP001
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Human GITR/TNFRSF18 Protein is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Gln26-Glu161 (UniProt accession: Q9Y5U5-1).
Product Details
- Molecular Aliases
- CD357; GITR; GITR-D; TNFRSF18; AITR
- Protein Length
- Gln26-Glu161
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 15.4 kDa. Due to glycosylation, the protein migrates to 25-28 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human GITR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%
The purity of Human GITR is greater than 95% as determined by SEC-HPLC.
Immobilized Human GITR Ligand Trimer, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human GITR, His Tag with the EC50 of 2.60μg/ml determined by ELISA (QC Test).
Immobilized Human GITR, His Tag at 0.5μg (100μl/Well) on the plate. Dose response curve for Anti-GITR Antibody, hFc Tag with the EC50 of 4.7ng/ml determined by ELISA.
Human GITR, His Tag captured on CM5 Chip via Anti-his antibody can bind Human GITR Ligand Trimer, hFc Tag with an affinity constant of 4.47 nM as determined in SPR assay (Biacore T200).
Background
GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation.GIRT is a receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.
References
- Knee D A, Hewes B, Brogdon J L. Rationale for anti-GITR cancer immunotherapy[J]. European Journal of Cancer, 2016, 67:1-10. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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