Cynomolgus GITR/TNFRSF18 Protein
Catalog No: GITR-CP001
- Species
- Cynomolgus
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Cynomolgus GITR/TNFRSF18 Protein is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Gln20-Pro156 (accession: XP_005545180.2).
Product Details
- Molecular Aliases
- CD357; GITR; GITR-D; TNFRSF18; AITR
- Protein Length
- Gln20-Pro156
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 15.87 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS, 8% trehalose (pH 7.4).
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Cynomolgus GITR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus GITR is greater than 95% as determined by SEC-HPLC.
Immobilized Cynomolgus GITR, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-GITR Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test).
Cynomolgus GITR Ligand, His Tag immobilized on CM5 Chip can bind Cynomolgus GITR, His Tag with an affinity constant of 0.21 μM as determined in SPR assay (Biacore T200).
Background
GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation.GIRT is a receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.
References
- Knee D A, Hewes B, Brogdon J L. Rationale for anti-GITR cancer immunotherapy[J]. European Journal of Cancer, 2016, 67:1-10. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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