Human GDNF Protein
Catalog No: GDNF-HP001
- Species
- Human
- Expression System
- E. coli
- Activity
- Activity verified
Product overview
Recombinant Human GDNF Protein is expressed in E. coli without tagIt contains Ser78-Ile211 (UniProt accession: P39905-1).
Product Details
- Molecular Aliases
- Glial cell line-derived neurotrophic factor; hGDNF; Astrocyte-derived trophic factor (ATF)
- Protein Length
- Ser78-Ile211
- Expression System
- E. coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 15.06 kDa same as Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human GDNF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human GDNF is greater than 95% as determined by SEC-HPLC.
Immobilized Human GDNF, No Tag at 1 μg/ml (100μl/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).
Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is 2‑12 ng/mL in the presence of Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera. (QC Test)
Background
Glial cell line-derived neurotrophic factor (GDNF) is a 134 amino acid protein belonging in the GDNF family ligands (GFLs). GDNF was originally isolated from rat glial cell lines and identified as a neurotrophic factor with the ability to promote dopamine uptake within midbrain dopaminergic neurons.
References
- PMID: 32897420; PMCID: PMC7529617.
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