Human Fas Ligand/TNFSF6 Protein
Catalog No: TNFSF6-HP001
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Human Fas Ligand/TNFSF6 Protein is expressed in HEK293 cells with a His tag at the N-terminus. It contains amino acid residues Pro134-Leu281 (accession: AAH17502.1).
Product Details
- Molecular Aliases
- APTL; CD95-L; Fas ligand; APT1LG1; TNFSF6; FASLG; ALPS1B; CD178; FASL; TNLG1A; CD95L
- Protein Length
- Pro134-Leu281
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 18 kDa. Due to glycosylation, the protein migrates to 20-35 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human Fas Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human Fas Ligand, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human Fas, hFc Tag with the EC50 of 75.0ng/ml determined by ELISA (QC Test).
Human Fas, His Tag immobilized on CM5 Chip can bind Human Fas Ligand, His Tag with an affinity constant of 19.45 nM as determined in SPR assay (Biacore T200).
Background
Fas ligand (FasL, CD95L) is a 40-kDa type II transmembrane protein that binds to Fas (CD95) receptors and promotes programmed cell death. Fas receptors are expressed at higher levels in many tumors than in normal cells. Somewhat paradoxically, elimination of Fas or FasL from tumors also leads to death induced by CD95 receptor/ligand elimination (DICE).
References
- Rieux-Laucat F, Magérus-Chatinet A, Neven B. The Autoimmune Lymphoproliferative Syndrome with Defective FAS or FAS-Ligand Functions. J Clin Immunol. 2018 Jul;38(5):558-568. doi: 10.1007/s10875-018-0523-x. Epub 2018 Jun 17. PMID: 29911256.
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