Human CXCL5 Protein
Catalog No: CXCL5-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
Product overview
Recombinant Human CXCL5 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the N-terminus. It contains amino acid residues Ala37-Asn114 (UniProt accession: P42830).
Product Details
- Molecular Aliases
- ENA78; SCYB5; ENA-78(1-78);; ENA78(1-78)
- Protein Length
- Ala37-Asn114
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 34.30 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human CXCL5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CXCL5 is greater than 95% as determined by SEC-HPLC.
Background
C-X-C motif chemokine ligand 5 (CXCL5), also known as epithelial neutrophil-activating peptide 78 (ENA-78), is a pivotal member of the ELR+ CXC chemokine family, characterized by a conserved glutamic acid-leucine-arginine motif. CXCL5 plays a central regulatory role in shaping the tumor immune microenvironment (TIME). Its expression is regulated by transcription factors (NF-κB, STAT3, SP1, p53), epigenetic modifications (DNA methylation, histone acetylation, microRNAs), and cytokines/growth factors (IL-1β, TNF, EGF).
References
- Yang X, Kuang H, Sun R, Ye L, Chen Y, Hu Z, Deng C, Li X, Zhang L, Wang Y, Tan X, Liu S, Shen Y, Wu K, Qu J, Wu J. The role of CXCL5 in tumor microenvironment: Regulatory mechanisms and therapeutic potential. Int J Cancer. 2026 Apr 15;158(8):2023-2042. doi: 10.1002/ijc.70296. Epub 2025 Dec 18. PMID: 41410416.
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