Human CD8 beta/CD8B Protein
Catalog No: CD8-HF002
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human CD8 beta/CD8B Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Leu22-Pro170 (UniProt accession: P10966-1).
Product Details
- Molecular Aliases
- CD8 alphaβ CD8A&CD8B;
- Protein Length
- Leu22-Pro170
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 42.72 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Human CD8 beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD8 beta is greater than 95% as determined by SEC-HPLC.
Immobilized Human CD8 beta, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD8 beta Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. (QC Test)
Background
CD8 on thymocytes and thymus-derived T cells consists of disulfide-linked CD8a and CD8b chains, which are transmembrane proteins, containing N-terminal Ig domains, extended and glycosylated hinge and stalk regions, and transmembrane and cytoplasmic portions. The cytoplasmic portion of CD8β, mainly due to its palmitoylation, mediates partitioning of CD8 in lipid rafts, where it efficiently associates with p56 lck. In addition, the cytoplasmic portion of CD8β mediates constitutive association of CD8 with TCR/CD3. The resulting TCR-CD8 adducts exhibit high affinity for major histocom-patibility complex (MHC)-peptide.
References
- Arcaro A, Grégoire C, Boucheron N, Stotz S, Palmer E, Malissen B, Luescher IF. Essential role of CD8 palmitoylation in CD8 coreceptor function. J Immunol. 2000 Aug 15;165(4):2068-76. doi: 10.4049/jimmunol.165.4.2068. PMID: 10925291.
- Arcaro A, Grégoire C, Bakker TR, Baldi L, Jordan M, Goffin L, Boucheron N, Wurm F, van der Merwe PA, Malissen B, Luescher IF. CD8beta endows CD8 with efficient coreceptor function by coupling T cell receptor/CD3 to raft-associated CD8/p56(lck) complexes. J Exp Med. 2001 Nov 19;194(10):1485-95. doi: 10.1084/jem.194.10.1485. PMID: 11714755; PMCID: PMC2193676.
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