Biotinylated Human CD8 alpha/CD8A Protein
Catalog No: CD8-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human CD8 alpha/CD8A Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ser22-Asp182 (UniProt accession: P01732-1).
Product Details
- Molecular Aliases
- CD8a; Lyt-2; CD8; Leu2; MAL; p32; CD8a molecule; OKT8 T-cell antigen; Lyt2
- Protein Length
- Ser22-Asp182
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 20.5 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human CD8 alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Biotinylated Human CD8 alpha, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test).
Background
CD8 alpha enhances the responses of antigen-specific CTL activated through TCR through binding MHC class I, favoring lipid raft partitioning of TCR, and inducing intracellular signaling. CD8 alpha is also found on dendritic cells and rat macrophages, but whether CD8 alpha enhances responses of a partner receptor, like TCR, to activate these cells is not known.
References
- Gibbings DJ, Marcet-Palacios M, Sekar Y, Ng MC, Befus AD. CD8 alpha is expressed by human monocytes and enhances Fc gamma R-dependent responses. BMC Immunol. 2007 Aug 1;8:12. doi: 10.1186/1471-2172-8-12. PMID: 17678538; PMCID: PMC2000912.
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