Human CD52 Protein
Catalog No: CD52-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
Product overview
Recombinant Human CD52 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Gly25-Ser36 (UniProt accession: P31358).
Product Details
- Molecular Aliases
- CD52 molecule; CD52; CDW52 antigen (CAMPATH-1 antigen); CDw52; He5; HEL-S-171mP; CAMPATH-1; CAMPATH1;CDW52 antigen (CAMPATH1 antigen); HEL-S171mP
- Protein Length
- Gly25-Ser36
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 28 kDa. Due to glycosylation, the protein migrates to 40-45 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human CD52 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD52 is greater than 95% as determined by SEC-HPLC.
Background
CD52, also known as CAMPATH-1 antigen, HE5, and gp20, is a cell surface glycoprotein that can be targeted to induce immune suppression by complement-mediated cell lysis. Mature human CD52 is a 12 amino acid peptide that is tethered to the cell surface with a GPI linkage.CD52 may play a role in carrying and orienting carbohydrate, as well as having a more specific role.
References
- Jiang L, Yuan C M, Hubacheck J, et al. Variable CD52 Expression in Mature T Cell and NK Cell Malignancies: Implications for Alemtuzumab Therapy[J]. British Journal of Haematology, 2009, 145(2):173-179. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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