Human CD2/SRBC Protein
Catalog No: CD2-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human CD2/SRBC Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Lys25-Asp209 (UniProt accession: P06729).
Product Details
- Molecular Aliases
- T11; CD2; SRBC; LFA-2; LFA-3 receptor;LFA2; LFA3 receptor
- Protein Length
- Lys25-Asp209
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 47.9 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human CD2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD2 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human CD58, His Avi tag (Cat. CD5-HM418B) at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human CD2, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA. (QC Test)
Immobilized Human CD2, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Siplizumab, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA.
Human CD58, hFc Tag immobilized on CM5 Chip can bind Human CD2, hFc Tag with an affinity constant of 7.38 nM as determined in SPR assay (Biacore T200).
Loaded Biotinylated Human CD58, His-Avi tag (Cat. CD5-HM418B) on Steptavidin-Biosensor can bind Human CD2, hFc Tag with an affinity constant of 6.46 nM as determined in BLI assay.
Background
The CD2 family of receptors is evolutionarily conserved and widely expressed on cells within the hematopoietic compartment. In recent years several new members have been identified with important roles in the immune system. CD2 family members regulate natural killer (NK) cell lytic activity and inflammatory cytokine production when engaged by ligands on tumor cells.
References
- Shao T, Shi W, Zheng JY, Xu XX, Lin AF, Xiang LX, Shao JZ. Costimulatory Function of Cd58/Cd2 Interaction in Adaptive Humoral Immunity in a Zebrafish Model. Front Immunol. 2018 May 31;9:1204. doi: 10.3389/fimmu.2018.01204. PMID: 29904386; PMCID: PMC5990624. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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