Biotinylated Human CD2/SRBC Protein
Catalog No: CD2-HB002
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human CD2/SRBC Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Lys25-Asp209 (UniProt accession: P06729).
Product Details
- Molecular Aliases
- T11; CD2; SRBC; LFA-2; LFA-3 receptor; LFA2; LFA3 receptor
- Protein Length
- Lys25-Asp209
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 24.15 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human CD2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CD2 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human CD2, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human CD58, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human CD2, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Siplizumab, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA.
Loaded Biotinylated Human CD2, His-Avi tag on Steptavidin-Biosensor can bind Human CD58, hFc Tag (Cat. CD5-HM208) with an affinity constant of 5.28 nM as determined in BLI assay.
Background
The CD2 family of receptors is evolutionarily conserved and widely expressed on cells within the hematopoietic compartment. In recent years several new members have been identified with important roles in the immune system. CD2 family members regulate natural killer (NK) cell lytic activity and inflammatory cytokine production when engaged by ligands on tumor cells.
References
- Shao T, Shi W, Zheng JY, Xu XX, Lin AF, Xiang LX, Shao JZ. Costimulatory Function of Cd58/Cd2 Interaction in Adaptive Humoral Immunity in a Zebrafish Model. Front Immunol. 2018 May 31;9:1204. doi: 10.3389/fimmu.2018.01204. PMID: 29904386; PMCID: PMC5990624.
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